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β-arrestin1 sgRNAs were cloned into a vector with a Neomycin marker, and 3xHA-FPR1, Cas9, and β-arrestin2 sgRNA-expressing cells were infected with a β-arrestin1 sgRNA to generate the double knockout cell line

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Additionally, investigations into NAD+'s role in hypertension models have indicated effects on blood pressure regulation and vascular function in research animals